Pan-Actin (D18C11) Rabbit mAb 8456S from Cell Signaling Technology

Product Specs
ItemPan-Actin (D18C11) Rabbit mAb
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number8456S
Quantity100 µl
ApplicationsW, IHC-P, IF-IC
ReactivityH, M, R, Mk
HostRabbit
ClonalityMonoclonal
Targetbeta-Actin;ACTA1 (alpha actin, skeletal muscle);ACTG1 (gamma actin cytoplasmic nonmuscle);ACTC1 (alpha cardiac actin);ACTA2 (alpha actin, smooth muscle);ACTG2 (gamma actin, cytoplasmic)
NCBI Full Gene Nameactin alpha 1, skeletal muscle, actin gamma 1, actin beta, actin alpha cardiac muscle 1, actin gamma 2, smooth muscle, actin alpha 2, smooth muscle
NCBI Gene AliasesACTA3, CMYP2B, VSCM, ACT, HEL-176, VSCM1, MPFD, ASD5, ASMA, NEM1, NEM2, NEM3, PS1TP5BP1, SHPM, BRWS1, CMH11, CFTDM, ACTSA, MMIHS5, DFNA26, DFNA20, CFTD1, ACTSG, LVNC4, ACTL3, CFTD, CMD1R, CMYP2C, ACTA, CMYP2A, ACTC, ACTE, ACTG
Research AreaCytoskeletal Signaling
IsotypeIgG
CloneD18C11
Molecular Weight45

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Cell Signaling Technology

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Danvers, Massachusetts 01923

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Phone: 877-616-CELL
Phone: 978-867-2388
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Website: http://www.cellsignal.com/

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(1) Polo-like kinase-1 mediates hepatitis C virus-induced cell migration, a drug target for liver cancerLife Science AllianceNovember 1, 2023Korri E El-Khobar, Enoch Tay, Eve Diefenbach, Brian S Gloss, Jacob George, Mark W DouglasWAVE-2 were purchased from Cell Signaling Technology (#3128, #4738, #2144, #4970, #3195, #9812, #4848, #8456, #9062, #4513, #2461, #4651, #5741, and #3659). PLK1 primary antibody used for immunofluorescence was

(2) A Standardized Botanical Composition Mitigated Acute Inflammatory Lung Injury and Reduced Mortality through Extracellular HMGB1 ReductionMoleculesSeptember 11, 2023Mesfin Yimam, Teresa Horm, Alexandria O’Neal, Ping Jiao, Mei Hong, Lidia Brownell, Qi Jia, Mosi Lin, Alex Gauthier, Jiaqi Wu, et al.overnight at 4 °C with anti-HO-1 (1:1000, #ab13248, Abcam, Cambridge, UK) and anti-pan-actin (1:1000, #8456, Cell Signaling) antibodies, diluted in 5% nonfat dry milk in TBST. After three washes with TBST, the

(3) Time-dependent effects of hypoxia on cell metabolism, signaling, and iron homeostasis in the hematopoietic progenitor model KG1aFEBS open bioJuly 1, 2023Nahoui-Zarouri I, Léger C, El Samra G, Cottet-Rousselle C, Moulis JM.Antigen,Provider,Cat. No Pan‐Actin,Cell Signaling Technologies,D18C11 #8456 β‐tubulin,,9F3 #2128 Lamin B1,,D9V6H #13435 p27 Kip1,,D69C12 #3686 cyclin D1,,92G2 #2978 HIF1α,,D1S7W

(4) GAT107-mediated α7 nicotinic acetylcholine receptor signaling attenuates inflammatory lung injury and mortality in a mouse model of ventilator-associated pneumonia by alleviating macrophage mitochondrial oxidative stress via reducing MnSOD-S-glutathionylationRedox BiologyApril 1, 2023Alex G. Gauthier, Mosi Lin, Sidorela Zefi, Abhijit Kulkarni, Ganesh A. Thakur, Charles R. Ashby, Lin L. Mantellanti-MnSOD antibody (1:1000, #AD-SOD-110, Enzo, New York, NY), and anti-pan-actin antibody (1:1000, #8456, Cell Signaling), diluted in 5% nonfat dry milk in TBST. After three washes with TBST, the membranes

(5) Cancer Cells Enter an Adaptive Persistence to Survive Radiotherapy and Repopulate TumorAdvanced science (Weinheim, Baden-Wurttemberg, Germany)March 1, 2023Zhao Y, Lu T, Song Y, Wen Y, Deng Z, Fan J, Zhao M, Zhao R, Luo Y, Xie J, et al.Santa Cruz Biotechnology sc‐515732), anti‐SNCG (1:100, Abcam 55 424), anti‐human pan Actin (1:1200, CST 8456), or anti‐CD44v6 (1:300, R&D systems) primary antibody following the manufacturer's instructions. Collection

(6) Extracellular vesicles of human diabetic retinopathy retinal tissue and urine of diabetic retinopathy patients are enriched for the junction plakoglo bin proteinFront Endocrinol (Lausanne)January 6, 2023Jason Mighty, Alfonso Rubio-Navarro, Cui Shi, Jing Zhou, Miguel Flores-Bellver, Søren Heissel, Onyekwere Onwumere, Linda Einbond, Rajendra Gharbaran, Daniel S. Casper, et al.antibodies were mouse anti-γ-catenin (Santa Cruz, sc-33634, 1:1000), rabbit anti-actin (Cell Signaling, #8456, 1:5,000), mouse anti-CD81 (Santa Cruz, sc-166028, 1:1000), rabbit anti-CD63 (Novus, nbp2-67425, 1:1000).

(7) Primary cilia suppress Ripk3-mediated necroptosisCell death discoveryDecember 2, 2022Kieckhöfer E, Slaats GG, Ebert LK, Albert MC, Dafinger C, Kashkar H, Benzing T, Schermer B.CST, 1:1000), Nphp1 (Homemade polyclonal rabbit, 1:1000), β-Tubulin (E7, DSHB, 1:500) or pan-actin (#8456, CST, 1:1000) at 4 °C overnight. Fluorescence-coupled secondary antibodies (anti-mouse IgG (H + L) IRDye

(8) TurboID functions as an efficient biotin ligase for BioID applications in Xenopus embryosDevelopmental BiologyDecember 1, 2022Charlotte R. Kanzler, Michael Donohue, Megan E. Dowdle, Michael D. Sheets3F10, Roche, 11867423001), anti-actin polyclonal antibody (1:1000-1:10000; Cell Signaling Technology 8456S), anti-actin monoclonal antibody (1:10000; Developmental Studies Hybridoma Bank, JLA20), or anti-alpha-tubulin

(9) Extracellular Vesicles from Human Umbilical Cord-Derived MSCs Affect Vessel Formation In Vitro and Promote VEGFR2-Mediated Cell SurvivalCellsNovember 24, 2022Muñiz-García A, Wilm B, Murray P, Cross MJ.(C67E7, #4691), phospho-ERK1/2 (p44/42 MAPK, 137F5, #4695), GAPDH (D16H11, #5174) and pan-Actin (D18C11, #8456) were from Cell Signalling Technology (CST; Danvers, MA, USA). The antibody anti-RCAN1 (#D6694) was

(10) RAD21 amplification epigenetically suppresses interferon signaling to promote immune evasion in ovarian cancerThe Journal of clinical investigationNovember 15, 2022Deng P, Wang Z, Chen J, Liu S, Yao X, Liu S, Liu L, Yu Z, Huang Y, Xiong Z, et al.The following antibodies were used for immunoblotting: anti-RAD21 (Abcam, ab992), anti-actin (CST, 8456S), anti-YAP (CST, 14074S), anti-TEAD4 (Abcam, ab97460), anti-FLAG (Sigma-Aldrich, F1804), anti-HDAC1

(11) Confined migration induces heterochromatin formation and alters chromatin accessibilityiScienceSeptember 16, 2022Hsia CR, McAllister J, Hasan O, Judd J, Lee S, Agrawal R, Chang CY, Soloway P, Lammerding J.1:1,000; mouse anti-GAPDH, Proteintech, 60004-1-Ig, 1:20,000; rabbit anti-pan-actin, Cell Signaling, 8456, 1:1,000) for 1 h at room temperature. Protein bands were detected using IRDye 680LT and IRDye 800CW

(12) Mechanical checkpoint regulates monocyte differentiation in fibrotic nichesNature MaterialsAugust 1, 2022Kyle H. Vining, Anna E. Marneth, Kwasi Adu-Berchie, Joshua M. Grolman, Christina M. Tringides, Yutong Liu, Waihay J. Wong, Olga Pozdnyakova, Mariano Severgnini, Alexander Stafford, et al.rabbit anti-NFKBIZ (1:100, Cell Signaling, #9244) and rabbit anti-pan-Actin (1:100, Cell Signaling, #8456). Gels stained with primary antibodies were washed twice with staining buffer for 30 min each at room

(13) Differential roles of RIG-I –like receptors in SARS-CoV-2 infectionbioRxivApril 18, 2022Duomeng Yang, Tingting Geng, Andrew G. Harrison, Penghua WangMethods Antibodies, cells and viruses The rabbit anti-MDA5 (Cat# 5321), RIG-I (Cat# 3743), and Actin (Cat# 8456) were purchased from Cell Signaling Technology (Danvers, MA, United States). Mouse anti-human MAVS (Cat#

(14) Sarm1 activation produces cADPR to increase intra-axonal Ca++ and promote axon degeneration in PIPNJournal of Cell BiologyFebruary 7, 2022Yihang Li et al.anti-IP3R1 (1:1,000; Thermo Fisher Scientific; PA1-901), and rabbit anti–pan-actin (1:1,000; Cell Signaling; 8456S). Bands were visualized with secondary antibodies conjugated to HRP (1:10,000; Bio-Rad; 1721019, 1706516)

(15) Branched-chain α-ketoacids are preferentially reaminated and activate protein synthesis in the heartNature CommunicationsDecember 1, 2021Jacquelyn M. Walejko et al.phospho-4E-BP1 (Thr 37/46) (Cell Signaling Technologies #2855), pan-actin (Cell Signaling Technologies #8456), puromycin (EMD Millipore #MABE343), β-tubulin (Sigma Aldrich, T8328), Alexa-Fluor Plus 800 (Thermo Fisher

(16) Overexpressing low-density lipoprotein receptor reduces tau-associated neurodegeneration in relation to apoE-linked mechanismsNeuronAugust 4, 2021Shi Y, Andhey PS, Ising C, Wang K, Snipes LL, Boyer K, Lawson S, Yamada K, Qin W, Manis M, et al.ab52818), Lgmn (Santa Cruz, Cat# sc-133234), p4EBP (Cell signaling, Cat# 2855), actin (Cell signaling, Cat# 8456). Lysosomal enzyme activity assay 10 mg of mouse brain cortex was homogenized in buffer containing

(17) ERK signaling mediates resistance to immunomodulatory drugs in the bone marrow microenvironmentScience AdvancesJune 4, 2021Jiye Liu et al.phosphor-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (no. 4370, Cell Signaling Technology), pan-actin (no. 8456, Cell Signaling Technology), NF-B1 p105/p50 (no. 3035, Cell Signaling Technology), lamin A/C (no. 4777, Cell Signaling Technology

(18) mTORC1 mediates fiber type-specific regulation of protein synthesis and muscle size during denervationCell Death DiscoveryJune 1, 2021Jae-Sung You, Kookjoo Kim, Nathaniel D. Steinert, Jie Chen, Troy A. Hornberger(T308, #9275), anti-Akt (#9272), anti-K48 linkage-specific polyubiquitin (#8081), anti-pan-actin (#8456), anti-phosphor-S6 (S240/244, #5364) from Cell Signaling Technology (Danvers, MA, USA); anti-ubiquitin

(19) Nox4 mediates skeletal muscle metabolic responses to exerciseMolecular metabolismMarch 1, 2021Specht KS, Kant S, Addington AK, McMillan RP, Hulver MW, Learnard H, Campbell M, Donnelly SR, Caliz AD, Pei Y, et al.Protein-Labeling Reagent (A44449, Invitrogen), Ponceau S (59803S, Cell Signaling Technologies) or pan-actin (mAb 8456, Cell Signaling Technologies). 2.8 Substrate oxidation GC were excised and washed in cold PBS. Approximately

(20) Cyclase-associated protein 2 (CAP2) controls MRTF-A localization and SRF activity in mouse embryonic fibroblastsScientific reportsFebruary 26, 2021Kepser LJ, Khudayberdiev S, Hinojosa LS, Macchi C, Ruscica M, Marcello E, Culmsee C, Grosse R, Rust MB.Proteintech), mouse anti-actin (1:5000, #NB600-535, Novus Biologicals), rabbit anti-pan-actin (1:5000, #8456S, Cell signaling), mouse anti-alpha-tubulin (1:2000, #T9026, Sigma) and mouse anti-GAPDH (1:10,000, #MAB5718,

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Pan-Actin (D18C11) Rabbit mAb

Pan-Actin (D18C11) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Pan-Actin (D18C11) Rabbit mAb from Cell Signaling Technology

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